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Bio-One

Information based on 2026 FDD
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Brand Highlights
  • Founded in 2006
  • Franchising Since 2011
  • 144 US Franchises
  • $135K - $221K Investment Range
  • $802K Average Gross Revenue
  • 7.5% Royalty Fee
  • $60K Franchise Fee
Brand Description

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

DID YOU KNOW?

How much does it cost to start a franchise with Bio-One?

$135K
$221K
Bio-One, a specialized biohazard remediation and crime scene cleanup franchise within the residential restoration sector, requires an initial investment ranging from $134,645 to $221,095. This investment supports the operational infrastructure necessary for emergency response capability, including commercial facilities for equipment storage, biohazard waste handling compliance, and specialized personal protective equipment inventory. The franchise fee was not disclosed in available materials.
Financial Summary
Franchise Fee
$60K
Investment Range
$135K - $221K
Investment Midpoint
$178K
Minimum Cash Required
$60K
Royalty Fees
7.5%
Brand Fund
2%
Brand Bragging Rights
18 years proven operational history since 2006
Specialized recession-resistant biohazard cleanup niche
18-33% lower investment than residential restoration sector average
Essential services with consistent insurance coverage support
Comprehensive training and certification programs for regulated industry
150 active units demonstrating system scalability and market demand
Financial Analysis
Bio-One operates in the specialized biohazard remediation and crime scene cleanup sector, positioned within the broader residential restoration industry. With 150 units across an 18-year operational history, the franchise demonstrates measured expansion consistent with a niche service requiring specialized training and regulatory compliance. The investment range of $134,645 to $221,095 is relatively accessible for home services franchises, though the specialized nature of biohazard cleanup creates operational barriers that limit competition while requiring franchisees to manage emotionally challenging work environments. The reported gross revenue of $802,263 suggests reasonable unit-level performance for a service business reliant on emergency response contracts, insurance coordination, and direct property owner engagement. This revenue level reflects both high per-job ticket values typical of specialized restoration work and the episodic demand patterns inherent to trauma scene and hoarding remediation services. Operational complexity centers on biohazard compliance, proper waste disposal, PPE inventory management, and 24/7 emergency availability. Scalability depends on building referral networks with emergency services, property managers, insurance adjusters, and healthcare facilities. The business model's resilience stems from consistent demographic demand (aging populations, accidental deaths, crime incidents) rather than discretionary consumer spending, though revenue volatility across territories may reflect geographic incident frequency and competitive intensity from regional restoration providers.
Expected Investment Range
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Average Gross Sales
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Financing
Financing Details

Franzy connects you with top-tier financing partners to help secure the funds to invest in a franchise like Bio-One. Whether you're looking for a loan or exploring other financial products, our partners provide expert guidance to ensure you obtain the necessary capital. They specialize in offering solutions tailored to the needs of franchisees, making the process of securing financing smooth and straightforward.

Why Financing with Franzy Partners?

Choosing to finance through Franzy's partners ensures you get the best terms and support for your franchise investment. Our partners have extensive experience in the franchising industry and offer specialized financial solutions tailored to your needs. With competitive interest rates and flexible repayment options, you can find the right financing plan that fits your budget and goals. Our partners are committed to providing personalized guidance throughout the financing process, making it easier for you to secure the necessary funds and confidently move forward with your franchise venture.

Finance Partners
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FranFund

Financing Partner

Tenet Financial Logo

Tenet Financial

Financing Partner

CRF USA Logo

CRF USA

Financing Partner

First Bank of the Lake Logo

First Bank of the Lake

Financing Partner

Live Oak Bank Logo

Live Oak Bank

Financing Partner

Pension Pros Logo

Pension Pros

Financing Partner

Guidant Financial Logo

Guidant Financial

Financing Partner

Preferred Funding Group Logo

Preferred Funding Group

Financing Partner

Location Analysis
Bio-One's geographic footprint concentrates in the Midwest, Southeast, and Mid-Atlantic regions, with particular density in Ohio, Florida, Pennsylvania, and Illinois. This distribution aligns strategically with markets offering aging populations, established homeownership rates, and sufficient population density to generate consistent biohazard remediation demand. The brand's presence in metropolitan markets like Columbus, Cincinnati, Tampa-St. Petersburg, and Charlotte reflects the need for both incident volume and established emergency services infrastructure to support referral generation. The franchise targets commercial/industrial facilities rather than retail locations, prioritizing rapid response capability over consumer visibility—a model well-suited to B2B relationships with property management firms, hospitality operators, and municipal agencies. Customer sentiment averaging 4.8-4.9 across review platforms is notably strong for a service category involving traumatic circumstances. Positive feedback emphasizes compassionate service delivery, rapid response, and thoroughness—attributes critical to reputation-dependent growth in a referral-driven sector. Negative themes around pricing transparency and insurance coordination suggest operational friction points common to emergency restoration services. The moderate review volume reflects the service's non-recurring nature and the discretion clients often prefer following traumatic incidents. Prospective investors should conduct territory-level due diligence assessing incident density, competitive saturation from regional restoration firms, and the strength of local emergency services relationships that drive sustainable lead generation.
Total US Locations150
Open Franchises144
Corporate Locations3
Average Sq. Foot150 square foot space in a small office building
Territory Map

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Franchise Net Unit Growth
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Additional Information

Executive Team

Get to know the leadership behind Bio-One. Learn about the experience and expertise of the executive team guiding Bio-One's success. For more details, refer to Item 2 of the Franchise Disclosure Document (FDD).

Litigation

Review any legal actions or pending litigation involving Bio-One. Understanding the legal history helps assess potential risks and the brand's business practices. For more details, refer to Item 3 of the Franchise Disclosure Document (FDD).

Bankruptcy

Review Bio-One's bankruptcy history and any filings by key personnel or affiliates. This critical information provides transparency about the brand's financial stability and management. For more details, refer to Item 4 of the Franchise Disclosure Document (FDD).

Franchisor Assistance

Learn about Bio-One's comprehensive support system for franchisees, including initial training programs and continuous operational assistance. Understanding the available resources and support structure is crucial for franchise success. For more details, refer to Item 11 of the Franchise Disclosure Document (FDD).

Frequently Asked Questions
Disclaimer

The information provided on this page is based on the latest Franchise Disclosure Document (FDD) we have on record, which was issued in 2026. This information is for informational purposes only and is not intended to constitute legal, financial, or business advice. We make no guarantees or claims regarding the completeness or accuracy. Only the franchisor can confirm that the information is complete and accurate and we recommend consulting the franchisor directly for the most recent FDD and regarding any questions that you may have about the information provided.

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