Bio-One
Home Services · Residential Restoration
Description
What is Bio-One?
Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.
- 18 years proven operational history since 2006
- Specialized recession-resistant biohazard cleanup niche
- 18-33% lower investment than residential restoration sector average
- Essential services with consistent insurance coverage support
- Comprehensive training and certification programs for regulated industry
- 150 active units demonstrating system scalability and market demand
Location Analysis
Where Bio-One wins
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Is your territory available?
Let's find out.
We'll take you through a few quick questions, then Bio-One confirms availability directly.
Financial Analysis
The numbers behind Bio-One
Financing partners
Vetted partners, tailored to franchisees
Your Franzy advisor can connect you with these partners later in the process — competitive rates, specialized in franchise financing.
FranFund
CRF USA
Nonprofit SBA lender; provides financing for franchise acquisitions, startups, and expansion.
First Bank of the Lake
Pension Pros
The model
How Bio-One works
Owner stays in an executive role — sets strategy, hires a manager, and oversees crews. Typically 5–20 hr/wk after ramp; many keep their day job.
Owner runs the business as their primary job — leads the team day-to-day on the ground, 40+ hr/wk.
FDD Item 7
Initial investment range
Per FDD Item 7, total initial investment ranges from $134,645 to $221,095. The midpoint $177,870 is what most franchisees report at signing — financing typically reduces cash-at-close by 80–90%. Knowing the investment range helps you plan confidently and ensure you're fully prepared to make the leap.
FDD Item 19
Average gross sales
According to Item 19 of the Franchise Disclosure Document, Bio-One has an average gross revenue of $385K. (Note: This information is based on the latest FDD in our records. Please review the Franchise Disclosure Document (FDD) and confirm this information directly with the brand. We make no claims of accuracy for the information presented.)
Growth over time
Franchise footprint
2026 Franchise Disclosure
FDD documents
Below are items 2, 3, 4, 7, 11 and 19 for Bio-One's 2026 FDD. The complete FDD is delivered to you directly by the franchisor, per the FTC Franchise Rule.
Connect to download Items 2, 3, 4, and 11 — direct from the franchisor.
Buyer FAQs
Frequently asked questions
The initial investment for a Bio-One franchise typically ranges between $134,645.00 and $221,095.00. This includes the franchise fee, equipment, real estate, and other startup costs. To get a detailed breakdown and better understand the financial requirements, we recommend scheduling a call with the Franzy team. We'll walk you through the specifics and answer any questions you might have. For more detailed information, refer to the financial sections of the FDD.
Disclaimer. The information provided on this page is based on the latest Franchise Disclosure Document (FDD) we have on record, which was issued in 2026. This information is for informational purposes only and is not intended to constitute legal, financial, or business advice. We make no guarantees or claims regarding the completeness or accuracy. Only the franchisor can confirm that the information is complete and accurate and we recommend consulting the franchisor directly for the most recent FDD and regarding any questions that you may have about the information provided.
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