Bio-One

Bio-One

Franzy VerifiedInformation based on 2026 FDD

Home Services · Residential Restoration

Investment min
$135K
Total: $135K–$221K
Avg gross revenue
$802K
Unit-level, 2026
Franchise fee
$60K
Per current disclosure
Royalty
7.5%
of gross revenue
Locations
144
Franchising since 2011

Description

What is Bio-One?

Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations in 45 states. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion. Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities. Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.

  • 18 years proven operational history since 2006
  • Specialized recession-resistant biohazard cleanup niche
  • 18-33% lower investment than residential restoration sector average
  • Essential services with consistent insurance coverage support
  • Comprehensive training and certification programs for regulated industry
  • 150 active units demonstrating system scalability and market demand

Location Analysis

Where Bio-One wins

Bio-One's geographic footprint concentrates in the Midwest, Southeast, and Mid-Atlantic regions, with particular density in Ohio, Florida, Pennsylvania, and Illinois. This distribution aligns strategically with markets offering aging populations, established homeownership rates, and sufficient population density to generate consistent biohazard remediation demand. The brand's presence in metropolitan markets like Columbus, Cincinnati, Tampa-St. Petersburg, and Charlotte reflects the need for both incident volume and established emergency services infrastructure to support referral generation. The franchise targets commercial/industrial facilities rather than retail locations, prioritizing rapid response capability over consumer visibility—a model well-suited to B2B relationships with property management firms, hospitality operators, and municipal agencies. Customer sentiment averaging 4.8-4.9 across review platforms is notably strong for a service category involving traumatic circumstances. Positive feedback emphasizes compassionate service delivery, rapid response, and thoroughness—attributes critical to reputation-dependent growth in a referral-driven sector. Negative themes around pricing transparency and insurance coordination suggest operational friction points common to emergency restoration services. The moderate review volume reflects the service's non-recurring nature and the discretion clients often prefer following traumatic incidents. Prospective investors should conduct territory-level due diligence assessing incident density, competitive saturation from regional restoration firms, and the strength of local emergency services relationships that drive sustainable lead generation.
Total US locations
150
Franchise units
144
Corporate locations
3
Avg. sq. footage

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Financial Analysis

The numbers behind Bio-One

Avg gross revenue$802,263
Investment range$134,645 – $221,095
Investment midpoint$177,870
Brand fund2%
Royalty7.5%
Franchise fee$60,000
Min. net worth$250,000
Min. liquid capital$60,000
Bio-One operates in the specialized biohazard remediation and crime scene cleanup sector, positioned within the broader residential restoration industry. With 150 units across an 18-year operational history, the franchise demonstrates measured expansion consistent with a niche service requiring specialized training and regulatory compliance. The investment range of $134,645 to $221,095 is relatively accessible for home services franchises, though the specialized nature of biohazard cleanup creates operational barriers that limit competition while requiring franchisees to manage emotionally challenging work environments. The reported gross revenue of $802,263 suggests reasonable unit-level performance for a service business reliant on emergency response contracts, insurance coordination, and direct property owner engagement. This revenue level reflects both high per-job ticket values typical of specialized restoration work and the episodic demand patterns inherent to trauma scene and hoarding remediation services. Operational complexity centers on biohazard compliance, proper waste disposal, PPE inventory management, and 24/7 emergency availability. Scalability depends on building referral networks with emergency services, property managers, insurance adjusters, and healthcare facilities. The business model's resilience stems from consistent demographic demand (aging populations, accidental deaths, crime incidents) rather than discretionary consumer spending, though revenue volatility across territories may reflect geographic incident frequency and competitive intensity from regional restoration providers.
Did you know? Bio-One, a specialized biohazard remediation and crime scene cleanup franchise within the residential restoration sector, requires an initial investment ranging from $134,645 to $221,095. This investment supports the operational infrastructure necessary for emergency response capability, including commercial facilities for equipment storage, biohazard waste handling compliance, and specialized personal protective equipment inventory. The franchise fee was not disclosed in available materials.

Financing partners

Vetted partners, tailored to franchisees

Your Franzy advisor can connect you with these partners later in the process — competitive rates, specialized in franchise financing.

FranFund

Lender

CRF USA

Nonprofit SBA lender; provides financing for franchise acquisitions, startups, and expansion.

Lender

First Bank of the Lake

Lender

Pension Pros

Lender

The model

How Bio-One works

01
Ownership
Part-Time (Executive)

Owner stays in an executive role — sets strategy, hires a manager, and oversees crews. Typically 5–20 hr/wk after ramp; many keep their day job.

Full-Time

Owner runs the business as their primary job — leads the team day-to-day on the ground, 40+ hr/wk.

02
Revenue
Revenue model not specified.
03
Customer
Customer model not specified.

FDD Item 7

Initial investment range

$135K–$221K
Most common
$134,645
Minimum
$177,870
Midpoint
$221,095
Maximum

Per FDD Item 7, total initial investment ranges from $134,645 to $221,095. The midpoint $177,870 is what most franchisees report at signing — financing typically reduces cash-at-close by 80–90%. Knowing the investment range helps you plan confidently and ensure you're fully prepared to make the leap.

FDD Item 19

Average gross sales

$500K$400K$300K$200K$100KN/A
$395K
$397K
$385K
2022
2023
2024
Avg
$392K
YOY change (2023 -> 2024)
-3%

According to Item 19 of the Franchise Disclosure Document, Bio-One has an average gross revenue of $385K. (Note: This information is based on the latest FDD in our records. Please review the Franchise Disclosure Document (FDD) and confirm this information directly with the brand. We make no claims of accuracy for the information presented.)

Growth over time

Franchise footprint

+2% YoY
1501209060300
2019
2020
2021
2022
2023
2024
130 units open as of 2026 FDD+3 in last 12 mo

2026 Franchise Disclosure

FDD documents

Below are items 2, 3, 4, 7, 11 and 19 for Bio-One's 2026 FDD. The complete FDD is delivered to you directly by the franchisor, per the FTC Franchise Rule.

Estimated initial investment
FDD Item 7 · PDF
Financial performance representations
FDD Item 19 · PDF
Members-only items
Executive team
FDD Item 2 · PDF
Litigation
FDD Item 3 · PDF
Bankruptcy
FDD Item 4 · PDF
Franchisor assistance
FDD Item 11 · PDF
Members only
Unlock the 2026 FDD

Connect to download Items 2, 3, 4, and 11 — direct from the franchisor.

Buyer FAQs

Frequently asked questions

The initial investment for a Bio-One franchise typically ranges between $134,645.00 and $221,095.00. This includes the franchise fee, equipment, real estate, and other startup costs. To get a detailed breakdown and better understand the financial requirements, we recommend scheduling a call with the Franzy team. We'll walk you through the specifics and answer any questions you might have. For more detailed information, refer to the financial sections of the FDD.

Disclaimer. The information provided on this page is based on the latest Franchise Disclosure Document (FDD) we have on record, which was issued in 2026. This information is for informational purposes only and is not intended to constitute legal, financial, or business advice. We make no guarantees or claims regarding the completeness or accuracy. Only the franchisor can confirm that the information is complete and accurate and we recommend consulting the franchisor directly for the most recent FDD and regarding any questions that you may have about the information provided.

Bio-One
Bio-One
$802K avg revenue · 144+ US franchises

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